ESR 1.1
Role of autophagy protein in the life cycle of mosquito-borne viruses

Project

The candidate will be supervised by excellent scientists from the autophagy (Reggiori) and the mosquito-borne viruses (Smit) research groups. Autophagy is an intracellular degradative process, which is part of our innate immunity, and several microbes have developed mechanisms to subvert it. The groups has explored the relevance autophagy proteins in the replication of various viruses and the candidate will focus on arboviruses including Dengue, West Nile, Zika and Chikungunya viruses. Using our siRNA library and virus strains expressing GFP, the candidate will determine in an automated manner which autophagy genes are relevant for the studied infections. The role of the hits in infections will be then studied in molecular detail using cell biological, biochemical, ultrastructural and microbiological methods. We expect to identify and characterize new factors involved in the life cycle of these human pathogens, with the ultimate goal of providing novel therapeutic targets for the treatment of their infections.

Supervisors

  • Prof. Dr. Fulvio Reggiori, Department of Cell Biology » Link
  • Prof. Jolanda Smit, Department of Medical Microbiology » Link

Collaborations

Karolinska Institute, Sweden

Relevant publications

  1. Reggiori F, Monastyrska I, Verheije MH, Cali T, Ulasli M, Bianchi S, et al. Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication. Cell Host Microbe. 2010 Jun 25;7(6):500–8.
  2. Monastyrska I, Ulasli M, Rottier PJM, Guan J-L, de Haan CAM, Reggiori F. An autophagy-independent role for LC3 in equine arteritis virus replication. Autophagy. 2013 Feb 1;9(2):164–74.
  3. Flipse J, Diosa-Toro MA, Hoornweg TE, van de Pol DPI, Urcuqui-Inchima S, Smit JM. Antibody-Dependent Enhancement of Dengue Virus Infection in Primary Human Macrophages; Balancing Higher Fusion against Antiviral Responses. Sci Rep. MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND: NATURE PUBLISHING GROUP; 2016 Jul;6.

Keywords

infection, autophagy, arboviruses, Dengue virus, Chikungunya virus, West Nile, Zika virus